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When Should Pipette Calibration Take Place?

By Edword Pitt Subscribe to RSS | December 7th 2011 | Views:

Good Laboratory Practices (GLP) should be kept in check for any laboratory environment. Within the laboratory, there are many precise fluid volumes that need to be analyzed for dilution or maybe transference of any kind. A pipette is the very helpful tool in that regard although GLP dictates that tools such as pipette should undergo proper calibration procedures. For this reason pipette calibration is a very vital thing for any laboratory test or measurement system.

Exactly what is a Pipette?

Simply, a pipette is a very important instrument in the lab used for transport a measured liquid volume. Pipettes are practically utilized in all major scientific labs for medical testing, molecular biology as well as analytical chemistry. The amounts of precision and moreover designs all fluctuate for pipettes depending on their use and also use. Various simple and so complex designs are out there. The most complex sorts of pipettes create a partial vacuum above the chamber that keeps the fluid. Micropipettes dispense only near 1 to 1000 µl while macro-pipettes present a much bigger quantity.

Sorts of Pipette

Various types of pipettes can be found including piston-driven air-displacement pipettes and positive-displacement pipettes which can be micropipettes; volumetric as well as graduated pipettes which are good sized capacity pipettes. Air-displacement pipettes have further more designs including flexible and / or fixed, volume handled, Single-channel, multi-channel or repeater, conical tips or maybe cylindrical tips, standard or simply locking and even manual or electronic. Positive-displacement pipettes are more uncommon and also utilized usually for crucial things like DNA. Mohr pipettes and serological or maybe blow-out pipettes are good examples of Graduated pipettes. Pipette calibration ranges from one pipette to another.

Pipette Calibration

To guarantee high quality specifications for a lab calibration of equipment is quite essential. It is a process by which the quality of a measuring device is often fine-tuned by comparison with a standard device. Any form of pipette calibration will give you assurance for the precise working of an device like pipette. It is a complicated problem to deal with due to the fact calibration actually depends on pipette models plus several other factors such as reliability and moreover precision of liquid volumes needed, operator training applications and several additional external factors. Many organizations gives their tools to a 3rd party calibrator.

When Have to Pipette Calibration Take Place?

Now when should you give your tool or maybe pipette in this case, for calibration? Well the solution to that isn't straightforward as really, it all depends on a lots of factors. The faster a pipette gets affected by inaccuracy the quicker it needs some kind of calibration and this period of time depends not simply on the make and even model of the pipette yet also on the training plus experience of the operator.

Many other factors that will have to be looked at is heat, moisture, heat transference, operator fatigue, form of liquid being distributed, precision as well as accuracy needed and moreover the amount of wear and tear inflicted on the pipette. All this collectively will identify when you opt for pipette calibration. In general, pipette calibration is needed every six months however calibration of equipment is needed every 4 months if the institution is regulated by GMP/GLP or FDA regulations.

Edword Pitt - About Author:
Edward Simpson works for a reputed equipment calibration firm. Apart from a worker he is a passionate writer and his articles focuses on topics around instrument calibration, flow calibration, pipette calibration and much more.

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